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Fitc-conjugated pac-1

WebApr 7, 2024 · And then, they were thoroughly washed, stained orderly with the vinculin antibody (1:500) overnight at 4 °C, the FITC-conjugated goat-anti-rabbit secondary antibody (1:5000) for 1 h, rhodamine-conjugated phalloidin (1:250) for 1 h, and DAPI (1:5000) for 5 min at room temperature. Webphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA).

FluoroTag™ FITC Conjugation Kit Sigma-Aldrich

WebConjugate: FITC. Order Details. View Available Conjugates. Catalog# & Conjugate Size Price; Alexa Fluor 350 NBP2-62201AF350: Alexa Fluor 405 NBP2-62201AF405 ... (PAC-1) [FITC] and receive a gift card or discount. Submit a Review. Bioinformatics. Gene Symbol: ITGA2B Popular Products ... WebFITC conjugates of antibodies, lectins, hormones, and growth factors have been used in a variety of immunohistochemical and flow cytometry applications. The protocols have been optimized for antibodies, but may be adapted to other proteins by the end user. philips handmixer hr 3740 https://theuniqueboutiqueuk.com

Fig. 1 a Anti-human fibrinogen antibody conjugated with FITC …

Weball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... WebThe binding of fibrinogen to the gpIIb/IIIa receptor is required for platelet aggregation. PAC-1 binds only to activated platelets and appears to be … WebThe PAC-1 Antibody FITC has been validated for the following applications: Flow Cytometry. Flow cytometry of PAC-1 in PHA-activated human peripheral blood. Sample was incubated with PAC-1 monoclonal antibody (Product # MA5-28564) conjugated to FITC. philips hanging sphere

Flow cytometric detection of activated mouse integrin

Category:FITC labeling of human insulin and transport of FITC

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Fitc-conjugated pac-1

FluoroTag™ FITC Conjugation Kit Sigma-Aldrich

WebMutated KRAS proteins are often constitutively active, resulting in the dysregulated growth and transformation pathways that promote pancreatic cancer growth, survival, and metastasis. Proteintech’s KRAS antibody is a favorite among researchers worldwide, cited in nearly 100 publications. Immunohistochemical analysis of paraffin-embedded ... WebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC …

Fitc-conjugated pac-1

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WebUse RGDS with conjugated PAC-1 antibody NOTE: Some commercial preparations can include lipopolysaccharide (LPS), a potent platelet activator. FACS brand flow cytometer. Refer to the appropriate instrument user’s guide for detailed information. ... stain with PAC-1 FITC/CD62P PE/CD61 PerCP in the presence of RGDS to inhibit PAC-1 binding ... WebAnti Pac1 Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results ... 1) Product Images from "Red blood cell-derived phosphatidylserine positive extracellular vesicles are associated with past ...

WebFeb 1, 2024 · Binding of the FITC-conjugated PAC-1 as well as the PE-conjugated anti-P-selectin was recorded as MFI measured on the PC beads minus MFI of the NC beads. After 6 months and 12 months a new batch of beads was used (indicated by the dashed lines). Data are presented as mean ± SD (n = 4) and linear regression lines are shown. ... WebKLH conjugated synthetic peptide derived from human AMPD3: Concentration - 100 μg (in 100 μl): 1 mg/ml: Form/Appearance: Lyophilized or Liquid: Conjugation: FITC: MW: 89kDa: UniProt ID: O08739, Q01432, O09178: Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

WebPac 1 Fitc Conjugate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebData on immunogenicity of adenovirus-vectored vaccine in chronic obstructive pulmonary disease (COPD) patients is limited. Therefore, we aimed to determine the humoral and cellular immune responses after homologous ChAdOx-1 vaccination in subjects with COPD. COPD subjects and age- and sex-matched healthy elderly receiving ChAdOx-1 …

WebIHC-plus™ ADCYAP1R1 / PAC1 Receptor Antibody LS-A109382 is a pathologist validated IHC antibody FITC Rabbit anti-Human ADCYAP1R1 / PAC1 Receptor. Validated for IHC. Tested on 20 paraffin-embedded human tissues. ... Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.) Purification.

WebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on … philips handmixer hr3741/00WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 … truthleadership gmail.comWebsupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1 philips hanfbachtalWebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. philips hand tool repairsWebCells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated ... truth law firmWebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and … truthleaksWeb(340507) BD™ FITC Mouse Anti-Human PAC-1 - BD Biosciences - CiteAb. 156 citations – A FITC-conjugated mouse monoclonal antibody, raised against Proteasome assembly chaperone 1 (Human), supplied by BD Biosciences. ... Conjugate FITC Target Proteasome assembly chaperone 1 (Human) UniProt O95456 - PSMG1_HUMAN. Gene PSMG1 … philips hannover