Phospho-upf1
WebSep 29, 2011 · SMG-1-mediated Upf1 phosphorylation takes place in the decay inducing complex (DECID), which contains a ribosome, release factors, Upf1, SMG-1, an exon junction complex (EJC) and a PTC-mRNA.... WebUpf1 contains a UPF1-type zinc-finger domain (aa 121-272) and a nucleotide-binding domain (aa 506-510). Specificity This rabbit polyclonal antibody detects Regulator of nonsense …
Phospho-upf1
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WebMay 29, 2010 · Compared with WT, GT tMEFs showed reduced levels of an anti–phospho-S/T-Q antibody–detected band comigrating with Upf1 in both the lysate and anti-Upf1 immunoprecipitate (Fig. 1E). This result confirms that disruption of Smg1 expression in Smg1 gt/gt embryos reduces the phosphorylation levels of Upf1. WebJul 3, 2024 · Phospho-UPF1 (p-UPF1) is a prominent marker of NMD substrates as its presence commits an RNA to degradation 17, 18, 41, 42. To identify NMD substrates in PEL cells we performed fRIP-coupled to...
WebAnti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at Ser1127 and has been tested in More>> Anti-phospho-Upf1 (Ser1127) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available … WebFeb 4, 2016 · UPF1 is a 130-kDa RNA helicase composed of two recombinase A (RecA)-like domains at its C terminus and an N-terminal regulatory domain 8, 9. In its closed conformation, an N-terminal...
WebAug 11, 2016 · Increase in phospho-UPF1 on ARE-mediated decay activation Given the observed hyperphosphorylation of UPF1 in response to mRNA decay factor depletion, we … Weblate UPF1 in vitr o and that the total cellular levels of phospho-UPF1 are diminished in cells that were shRNA-depleted for ATR.5 It will be important to map the UPF1 residues that are substrates for the different PIKKs and to verify to which extent these residues overlap. An appealing possibility is that different phopshorylation sites are
WebDec 14, 2024 · While preclearing each lysate, prepare the antibody−Dynabeads Protein A (see Note 10) by adding 5–10 μg of anti-phospho-UPF1 (Ser1116) antibody or, as a negative control, rabbit IgG (see Note 11) to 50 μl of washed Dynabeads Protein A from Subheading 3.3, step 1 in 300 μl of Hypotonic Gentle Lysis Buffer containing 150 mM NaCl (see Note …
WebApr 18, 2008 · Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay In mammalian cells, nonsense-mediated mRNA decay (NMD) … highclere parish councilWebThe UPF1 gene has been cloned and sequenced and shown to be (1) nonessential for viability, (2) capable of encoding a 109-kDa protein with both zinc finger and nucleotide (GTP) binding site motifs, and (3) partially homologous to the yeast SEN1 gene ( … how far is warfield va from newport news vaWebSep 1, 2006 · Since the phosphorylation of Upf1 by SMG-1 is a critical rate-limiting step for NMD, the decrease in phospho-Upf1 suggested that cellular NMD is suppressed. FIG. 2 Knockdown of Upf1 or SMG-1 by synthetic siRNA inhibits NMD, resulting in the accumulation of PTC-containing COL α2 (VI) mRNA and collagen VI protein. (A) Synthetic siRNA … how far is warner robins from atlantaWebApr 6, 2024 · Phospho-UPF1 recruits SMG6, which cleaves the mRNA close to the PTC, SMG5-7, which recruits CCR4-Not deadenylase and the decapping enzyme Dcp2 (reviewed in ref. ) . However, how the C-terminally truncated proteins, which are encoded by nonsense mRNA and which are potentially harmful for the cell, are recognized and efficiently … how far is warrensburg from kansas cityWebAnti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at Ser1127 and has been tested in Immunoprecipitation, peptide … highclere pendantWebSep 2, 2024 · DDIT4-AS1 silencing inhibited stemness and enhanced chemosensitivity to GEM (Gemcitabine). Mechanistically, DDIT4-AS1 promoted the phosphorylation of UPF1 by preventing the binding of SMG5 and PP2A to UPF1, which decreased the stability of the DDIT4 mRNA and activated the mTOR pathway. how far is warrenton va from washington dcWebSep 29, 2011 · SMG-1-mediated Upf1 phosphorylation takes place in the decay inducing complex (DECID), which contains a ribosome, release factors, Upf1, SMG-1, an exon junction complex (EJC) and a PTC-mRNA. However, the significance and the consequence of Upf1 phosphorylation remain to be clarified. highclere partners llp