WebThe pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the … The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes Support How can we help? Choose an option. Contact Us
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WebNov 24, 2016 · The host-plasmids system used for the production of hGH was pTrcHis/ZRG (pTrcHis Topo, Invitrogen, USA) consists of the structural gene for rhGH, trc promoter for high-level expression in E. coli, the lacO sequence for induction by IPTG and 6x His tag for purification of the recombinant protein. All experiments were repeated at least three times. WebNov 22, 2024 · The FlaA and FlaB genes were amplified by PCR, cloned into pTrcHis-TOPO (Thermo Fisher Scientific), and expressed in E. coli BL21 competent cells (New England Biolabs) for production and purification of 6XHis-tagged rFlaA and rFlaB proteins 54. The 6X-His-FlaA and FlaB proteins were purified on a nickel-nitrilotriacetic acid affinity column ... WebPrototroph definition, a microorganism that has the same nutritional requirements as the parent organism. See more. shriram motor finance