Shscr-pgywlaj
WebPlasmid pLKO.1 shSCR from Dr. Sheila Stewart's lab contains the insert shSCR and is published in Curr Biol. 2008 Apr 8. 18(7):496-500. This plasmid is available through … WebFigure 1B demonstrates that silencing of SDF-1 resulted in decreased mRNA in cells, and decreased SDF-1 protein secreted into cell supernatants of MSCs (shSDF-1) compared with a scrambled control (shSCR). shSDF-1 and shSCR MSCs were next phenotyped using flow cytometry. shSDF-1 and shSCR MSCs showed comparable expression of mesenchymal …
Shscr-pgywlaj
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WebJan 4, 2024 · For tumor metastasis assay, 5 × 10 5 CFPAC-1 cells with sh-circRTN4 or shSCR were resuspended in 1× PBS with 20% matrigel and were injected orthotopically to the head of the pancreas of randomized male BALB/c nude mice aged 4 to 6 weeks (four mice per group) . The establishment and growth of tumor were monitored every 3–4 days. WebApr 3, 2024 · Introduction. RNA interference is a widely used tool to reduce the expression of mRNAs. RNAi is initiated by double-stranded (ds)RNAs or pre-microRNAs, which are cleaved by Dicer, an RNase III enzyme, producing 21–23 nucleotide short interfering (si)RNAs or micro (mi)RNAs respectively, containing 2nt 3’ overhangs Citation 1, Citation 2.The …
WebMay 1, 2015 · We examined the effect of specific and local silencing of sodium/hydrogen exchanger isoform 1 (NHE1) with a small hairpin RNA delivered by lentivirus (L-shNHE1) in the cardiac left ventricle (LV) wall of spontaneously hypertensive rats, to reduce cardiac hypertrophy. Thirty days after the lentivirus was injected, NHE1 protein expression was … WebSep 5, 2024 · Results and discussion. To examine the effect of over-nutrition on SWELL1 expression in adipose tissue depots and in liver we performed intravenous injection of a novel adeno-associated virus serotype (AAV/rec2) Citation 10 expressing either a scrambled short-hairpin RNA (shSCR) or an shRNA-directed to SWELL1 (shSWELL1), both co …
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